Journal: Frontiers in Neuroscience
Article Title: Local arrangement of microfibrillar-associated protein 5 with neurovascular and extracellular components in non- and ischemia-affected brain regions of mice
doi: 10.3389/fnins.2025.1593948
Figure Lengend Snippet: Multiple immunofluorescence labeling of the microfibrillar-associated protein 5 (MFAP5) together with representative structures from neurons (Neuronal Nuclei, NeuN; human protein C and d, HuC/D), oligodendrocytes (myelin basic protein, MBP), microglia (ionized calcium-binding adapter molecule 1, Iba1), and astroglia (aquaporin 4, Aqp4; glial fibrillary acidic protein, GFAP) in neocortical brain regions in mice, affected by focal cerebral ischemia for 24 h. For each combination of markers, the local arrangement is compared between the non-affected (A,C,E) and the ischemia-affected hemisphere (B-B”,D-D”’,F-F”’) . Scale bars are labeled in related figures, while the scale bar in (B”) is also valid for (B’) , and the scale bars in (D”’,F”’) are also valid for (D’,D”,F’,F”) . Meanings of asterisk, arrows, and arrowheads are described in the main text.
Article Snippet: Ionized calcium-binding adapter molecule 1 , Iba1 , 1:200 , 234004 , Synaptic Systems , Donkey anti-guinea pig Cy5.
Techniques: Immunofluorescence, Labeling, Binding Assay